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Three plasmids (pGL1124, pGL1224 and pGL1217) were being made to enable the alternative of 1 allele of CYC9Lister 427 cells. Trypanosomes were being transfected with Every plasmid individually, and then subjected to your 2nd transfection with on the list of other plasmids, with another resistance gene, with all pairwise mixtures executed.Good self